molecular cloning and expression of the leishmania infantum kmp-11 gene
Authors
abstract
conclusions the results of the present study will increase our knowledge about molecular cloning and expression of the l. infantum kmp-11 gene, and this may be used as an effective target for controlling visceral leishmaniasis. results the kmp-11 gene was successfully subcloned in pcdna3 as a eukaryotic expression vector. recombinant kmp-11 protein was confirmed by the reverse transcriptase polymerase chain reaction (rt-pcr) method. materials and methods dna was extracted from l. infantum (mhom/tn/80/ipi1), and amplified by pcr and a specific primer. afterwards the purified pcr products were successfully ligated into the ptz57r/t plasmid vector. the pcdna3 plasmid was used as an expression vector and the kmp-11 gene subcloned into this plasmid. the ptkmp-11 plasmid was digested by restriction enzymes to confirm cloning of this gene in the ptz57r/t plasmid. in the last step, the subcloned gene was expressed in a eukaryotic cell. objectives in the present study, the kmp-11 gene was extracted from leishmania infantum and then, cloned and expressed in an expression vector.the main objective of this study was to provide an introduction to dna vaccine production against visceral leishmaniasis. background visceral leishmaniasis (kala-azar) is one of the most serious tropical diseases, and it can lead to death. the kinetoplastid membrane protein-11 (kmp-11) is highly conserved in all stages of the leishmania life cycle.
similar resources
Molecular Cloning and Expression of the Leishmania infantum KMP-11 Gene
Background: Visceral leishmaniasis (Kala-azar) is one of the most serious tropical diseases, and it can lead to death. The kinetoplastid membrane protein-11 (KMP-11) is highly conserved in all stages of the Leishmania life cycle. Objectives: In the present study, the KMP-11 gene was extracted from Leishmania infantum and then, cloned and expressed in an expression vector.The main objective of t...
full textcloning and expression of leishmania infantum lpg3 gene by the lizard leishmania expression system
background: various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. in the present study, we used a new protein expression system based on the iranian lizard leishmania, a trypanosomatid protozoan as a host, for the expression of lpg3 gene from leishmania infantum (l.infantum). methods: the lpg3 gene was cloned in the expression cass...
full textCloning and Expression of Leishmania infantum LPG3 Gene by the Lizard Leishmania Expression System
BACKGROUND Various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. In the present study, we used a new protein expression system based on the Iranian Lizard Leishmania, a trypanosomatid protozoan as a host, for the expression of LPG3 gene from Leishmania infantum (L.infantum). METHODS The LPG3 gene was cloned in the expression cass...
full textMolecular Cloning and Characterization of P4 Nuclease from Leishmania infantum
Parasite of the genus Leishmania is reliant on the salvage pathway for recycling of ribonucleotides. A class I nuclease enzyme also known as P4 nuclease is involved in salvage of purines in cutaneous Leishmania species but the relevant enzymes have not been characterized in Leishmania infantum (L. infantum). The aim of this study was to clone and characterize the gene encoding class I nuclease ...
full textcloning and expression of recombinant plasmid containing p36/lack gene of leishmania infantum iranian strain.
background : there are several methods, such as vaccination, to control visceral leishmaniasis. although there is no efficient vaccine, it seem dna vaccination with stimulates both cellular and humoral immunity apparently is the best way. the aim of this study was cloning and expression of lack gene, a 36kd protein, as a can-didate protein for vaccination against iranian l.infantum. methods: ir...
full textCloning and Expression of Recombinant Plasmid Containing P36/LACK Gene of Leishmania infantum Iranian Strain
BACKGROUND There are several methods, such as vaccination, to control visceral leishmaniasis. Although there is no efficient vaccine, it seem DNA vaccination with stimulates both cellular and humoral immunity apparently is the best way. The aim of this study was cloning and expression of LACK gene, a 36kD protein, as a candidate protein for vaccination against Iranian L. infantum. METHODS Ira...
full textMy Resources
Save resource for easier access later
Journal title:
jundishapur journal of microbiologyجلد ۶، شماره ۲، صفحات ۰-۰
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023